human chondrosarcoma cell lines sw1353  (ATCC)

Journal: Discover Oncology

Article Title: Mendelian randomization analysis of causal relationships between chondrosarcoma and angiogenesis related genes

doi: 10.1007/s12672-026-04621-0

Figure Lengend Snippet: RT-qPCR Validation of Key Genes in Chondrosarcoma Cell Lines. (A-D) Relative expression levels of LINC00984, TXNDC2, MPL28, and RP11-474N24.6 in SW1353, JJ012, and OUMS-27 chondrosarcoma cell lines compared to normal C28/I2 chondrocytes. Data represent mean ± SD from three independent experiments. *** P < 0.001, ** P < 0.01 vs. control group

Article Snippet: Human chondrosarcoma cell lines SW1353, JJ012, and OUMS-27, along with normal human chondrocyte cell line C28/I2 (control), were obtained from ATCC and cultured in DMEM supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37 °C with 5% CO2.

Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Control

Journal: Scientific Reports

Article Title: SOX9 represses the human galectin-3 promoter in SW1353 cells: potential implications for osteoarthritis

doi: 10.1038/s41598-026-50507-0

Figure Lengend Snippet: ( a ) The LGALS3 full-length promoter (−2638/+52) and the deletions (−1925/+52), (−1442/+52), (−535/+52), (−428/+52), (−328/+52), (−228/+52), (−117/+52), (−97/+52), (−77/+52), (−37/+52), (−17/+52) are shown in a diagram drawn to scale. Positions are numbered according to the distance to the transcription start site (TSS), where negative means upstream and positive means downstream of the TSS. ( b ) Relative activity of LGALS3 promoter sequence deletions in SW1353 cells. The activity of these deletions is shown as an x-fold increase relative to cells transfected with the promoter-less vector pGL4.20 (Basic). The graph shows the results’ mean ± SD of at least four biological replicates, each including three technical replicates, with error bars representing the standard deviation. * p < 0.05.

Article Snippet: SW1353 human chondrosarcoma cells (ATCC HTB-94TM, American Type Culture Collection (ATCC)) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 in Dulbecco’s Modified Eagles Medium (DMEM), containing 10% FCS and 1% penicillin/streptomycin/L-glutamine.

Techniques: Activity Assay, Sequencing, Transfection, Plasmid Preparation, Standard Deviation

Journal: Scientific Reports

Article Title: SOX9 represses the human galectin-3 promoter in SW1353 cells: potential implications for osteoarthritis

doi: 10.1038/s41598-026-50507-0

Figure Lengend Snippet: Relative activity of the LGALS3 full-length promoter (−2638/+52) in SW1353 cells when co-transfected with 30 ng of different SOX-containing plasmids (pcDNA3.1(+)-SOX2, -SOX4, -SOX5, -SOX6 and -SOX9). The relative activity of the promoter in the different conditions is calculated as a ratio to the promoter activity of the cells transfected with the vector pcDNA3.1(+) lacking the respective SOX. The graph shows results’ mean relative activity ± SD from four technical replicates, each including three biological replicates, with error bars representing the standard deviation. * p < 0.05.

Article Snippet: SW1353 human chondrosarcoma cells (ATCC HTB-94TM, American Type Culture Collection (ATCC)) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 in Dulbecco’s Modified Eagles Medium (DMEM), containing 10% FCS and 1% penicillin/streptomycin/L-glutamine.

Techniques: Activity Assay, Transfection, Plasmid Preparation, Standard Deviation

Journal: Scientific Reports

Article Title: SOX9 represses the human galectin-3 promoter in SW1353 cells: potential implications for osteoarthritis

doi: 10.1038/s41598-026-50507-0

Figure Lengend Snippet: Relative activity of the LGALS3 full-length promoter (−2638/+52) in SW1353 cells when titrated with different amounts of SOX-containing plasmids. ( a ) pcDNA3.1(+)-SOX2, ( b ) pcDNA3.1(+)-SOX4, ( c ) pcDNA3.1(+)-SOX5, ( d ) pcDNA3.1(+)-SOX6, ( e ) pcDNA3.1(+)-SOX9. The amounts of DNA used were: 0.1 ng, 1 ng, 10 ng, 100 ng. For each condition, a negative control without the SOX-encoding plasmid was included. The x-axis is plotted on a logarithmic scale. The relative activity of the promoter in the different conditions is calculated as a ratio to the promoter activity of the cells transfected with the vector pcDNA3.1(+) lacking the respective SOX. The graph shows results’ mean relative activity ± SD from three biological replicates, including three technical replicates, with error bars representing the standard deviation. * p < 0.05.

Article Snippet: SW1353 human chondrosarcoma cells (ATCC HTB-94TM, American Type Culture Collection (ATCC)) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 in Dulbecco’s Modified Eagles Medium (DMEM), containing 10% FCS and 1% penicillin/streptomycin/L-glutamine.

Techniques: Activity Assay, Negative Control, Plasmid Preparation, Transfection, Standard Deviation

Journal: Scientific Reports

Article Title: SOX9 represses the human galectin-3 promoter in SW1353 cells: potential implications for osteoarthritis

doi: 10.1038/s41598-026-50507-0

Figure Lengend Snippet: Relative activity of LGALS3 promoter deletion variants in SW1353 cells when co-transfected for 24 h with SOX-protein-containing plasmids: ( a ) SOX2 or ( b ) SOX9. The graph shows results’ mean ± SD from at least three biological replicates, including three technical replicates, with error bars representing the standard deviation. The white bars represent the negative control (empty pcDNA3.1(+)). ( a ) The light grey bars represent SOX2. ( b ) The dark grey bars represent SOX9.

Article Snippet: SW1353 human chondrosarcoma cells (ATCC HTB-94TM, American Type Culture Collection (ATCC)) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 in Dulbecco’s Modified Eagles Medium (DMEM), containing 10% FCS and 1% penicillin/streptomycin/L-glutamine.

Techniques: Activity Assay, Transfection, Standard Deviation, Negative Control

Journal: Scientific Reports

Article Title: SOX9 represses the human galectin-3 promoter in SW1353 cells: potential implications for osteoarthritis

doi: 10.1038/s41598-026-50507-0

Figure Lengend Snippet: Fold enrichment of specific promoter binding of HaloTag-SOX9 and hGal3p(−93/+49). ( a ) HaloChIP TM experiments were performed using SW1353 cells transfected with HaloTag-SOX9 or with the HaloTag-only vector (pHTN, control). DNA bound to SOX9 was isolated using the HaloCHIP™ system (Promega), and DNA was amplified via quantitative PCR. ADAMTS-4 is a known target of SOX9 and was used as a positive control. The graph shows mean ± SD from two biological replicates, with error bars representing the standard deviation. White bars represent the HaloTag-only control (pHTN). The dark grey bars represent HaloTag-SOX9. ( b ) The sequence stretch studied is shown, with a potential SOX9 binding site shown in a box. ( c ) A canonical SOX9 binding motif is shown.

Article Snippet: SW1353 human chondrosarcoma cells (ATCC HTB-94TM, American Type Culture Collection (ATCC)) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 in Dulbecco’s Modified Eagles Medium (DMEM), containing 10% FCS and 1% penicillin/streptomycin/L-glutamine.

Techniques: Binding Assay, Transfection, Plasmid Preparation, Control, Isolation, Amplification, Real-time Polymerase Chain Reaction, Positive Control, Standard Deviation, Sequencing

Journal: Scientific Reports

Article Title: SOX9 represses the human galectin-3 promoter in SW1353 cells: potential implications for osteoarthritis

doi: 10.1038/s41598-026-50507-0

Figure Lengend Snippet: Mutagenesis of the predicted SOX9 binding-site in the LGALS3 promoter. ( a ) The predicted SOX9 motif is shown within the wild-type sequence (WT); Mut shows the mutated motif. ( b ) Firefly luciferase activity (Firefly/ Renilla ratio) of the LGALS3 promoter construct (−97/+52) WT versus the LGALS3 promoter construct (−97/+52) carrying a mutation in the predicted SOX9 binding site in SW1353 cells, co-transfected with pcDNA (white bars) or pcDNA- SOX9 (grey bars). Data represent mean ± SD of three independent experiments performed in triplicate. Statistical significance is indicated as * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: SW1353 human chondrosarcoma cells (ATCC HTB-94TM, American Type Culture Collection (ATCC)) were cultured at 37 °C in a humidified atmosphere with 5% CO 2 in Dulbecco’s Modified Eagles Medium (DMEM), containing 10% FCS and 1% penicillin/streptomycin/L-glutamine.

Techniques: Mutagenesis, Binding Assay, Sequencing, Luciferase, Activity Assay, Construct, Transfection

Journal: Bioresources and Bioprocessing

Article Title: Ulvan and Ulva oligosaccharides from Ulva sp. attenuate osteoarthritis in a high-fat diet and ligamentous meniscal injury-induced rat model

doi: 10.1186/s40643-026-01012-9

Figure Lengend Snippet: Effects of UE and UH on the production of IL-6, MMP-3, and Collagen 2α1 in MIA-induced SW1353 cells. Data are presented as mean ± SD ( n = 3). # indicates significant difference at p < 0.05; ## indicates significant difference at p < 0.01. Collagen 2α1, Collagen Type II Alpha 1; IL, interleukin; MIA, monosodium iodoacetate; MMP, matrix metalloproteinase; UE, Ulva extract; UH, Ulva hydrolysate

Article Snippet: The human chondrosarcoma cell line SW1353 was purchased from the Bioresource Collection and Research Center of the Food Industry Research and Development Institute and the American Type Culture Collection (ATCC).

Techniques: